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Polyscience，Inc，成立于1961年，起家于化學(xué)但超越化學(xué)領(lǐng)域的美國(guó)制造商，總部位于賓夕法尼亞州沃靈頓，公司業(yè)務(wù)主要涵蓋三大塊：1.實(shí)驗(yàn)用產(chǎn)品：a）成千上萬(wàn)種具品牌特色的化合物和試劑，應(yīng)用于實(shí)驗(yàn)室研究，組織學(xué)，電鏡和解剖病理學(xué)以及其他診斷和生命科學(xué)研究；b）種類極其寬廣的單體和聚合體，滿足各方研究領(lǐng)域；c）有機(jī)/無(wú)機(jī)化合物，生物可降解材料，磁珠，熒光素，染料和特定抗體，以及常規(guī)蛋白包被顆粒（直徑范圍40nm~10mm）的首要制造工廠；d）頂端多聚化合物的供貨中心，滿足各種高性能工業(yè)領(lǐng)域需求，包括替代能源，汽車電子，國(guó)防&電子，便攜式通信和計(jì)算機(jī)，醫(yī)療器械組裝。2. 定制合成；3.cGMP生產(chǎn)服務(wù)。：1（800）523-2575；（215）343-6484。
Brand Story：
Me，Polyscience，Inc，Chemistry beyond the ordinary
Me，Polyscience，Inc，Headquartered at Warrington，PA，Call vial 1（800）523-2575
Me，Polyscience，Inc，Manufacturing hundreds of specialty chemicals and reagents for laboratory research, Histology, Light Microscopy and Anatomic Pathology as well as other clinical and life science applications.
Me，Polyscience，Inc，Stocking a wide portfolio of monomers and polymers for research applications.
Me，Polyscience，Inc，Developed to be the primary source for organic, inorganic, biodegradable, magnetic, fluorescent, dyed and specific antibody and general protein coated particles in diameters spanning the range of 40nm to 10mm.
Me，Polyscience，Inc，Offering innovative polymer products to meet the high performance requirements of a variety of industries including alternative energy, automotive electronics, defense & aerospace electronics, portable communications & computing, medical device assembly, and more.
——Written/Edited by V. Shallan
Special Products：
你會(huì)喜歡的第二代聚合物轉(zhuǎn)染試劑
Polyethylenimine，Linear，Mw 25，000 線性化聚乙烯亞胺（PEI 25，000）
基本描述：
線性化聚乙烯亞胺PEI 25，000，一種高電荷陽(yáng)離子聚合物，非常容易結(jié)合于高電荷陰離子基質(zhì)。工業(yè)應(yīng)用上，線性化PEI可改善負(fù)電荷染料的物理外觀，以調(diào)整染料的化學(xué)特性和增強(qiáng)染料與固相基質(zhì)的黏附能力，常用作黏附增強(qiáng)劑，作用同多聚賴氨酸（poly-lysine）；科研應(yīng)用上，線性化PEI能與DNA或其他負(fù)電荷生物大分子簡(jiǎn)單結(jié)合，正是這一特性，使得成為一種非常行之有效的載體運(yùn)輸介質(zhì)（Vector carrier），即轉(zhuǎn)染試劑。
化學(xué)性質(zhì)：
CAS NO. | 9002-98-6, 26913-06-4 |
Formula | (CH2CH2NH)n |
Molecular Weight | 25,000 |
Melting Point | 73-75º |
Solubility | Soluble In: hot water, cold water at low pH, methanol, and ethanol. Insoluble In: benzene, ethyl ether and acetone |
Appearance | White to yellow solid |
訂購(gòu)信息：【*，現(xiàn)貨供應(yīng)】
品牌 | 貨號(hào) | 名稱 | 規(guī)格 | *（元） |
Polysciences | 23966-2 | 線性聚乙烯亞胺（MW 25，000） | 2g | 2684 |
另，提供更高轉(zhuǎn)化效率的PEI Max 40，000，自由基形式分子量相當(dāng)于PEI 25，000，研究證實(shí)其體外質(zhì)粒DNA轉(zhuǎn)染效率提高達(dá)21倍。 | ||||
Polysciences | 24765-2 | 線性聚乙烯亞胺鹽酸鹽Max，（MW 40，000） (Equivalent to Mw 25,000 in Free Base Form) | 2g | 3184 |
使用方法【來(lái)源于：CSH protocols，僅作交流學(xué)習(xí)】 ：
1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.
2. Add 500 mg of PEI to the beaker with stirring.
3. Add concentrated HCl drop-wise to the solution to pH <2.0.
4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.
Approximay 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.
5. Add concentrated NaOH drop-wise to the solution to pH 7.0.
Approximay 500 μL of 10 M NaOH will be required to neutralize the PEI solution.
6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.
7. Filter-sterilize the solution through a 0.22-μm membrane.
8. Store aliquots of the desired volume at -20°C.
應(yīng)用文獻(xiàn)【基因轉(zhuǎn)染用途】 ：
Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.
Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pin (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98.
Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.
——Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
更的PEI聚乙烯亞胺聚合物轉(zhuǎn)染試劑
Polyethylenimine HCl MAX, Linear, Mw 40,000
線性化聚乙烯亞胺鹽酸鹽 MAX（PEI 40，000）
基本描述：
Polyethylenimine HCl MAX, Linear, Mw 40,000（PEI 40，000）是熱賣產(chǎn)品線性化聚乙烯亞胺PEI25,000的升級(jí)產(chǎn)品，不同之處在于1）*水解（去乙酰化）；2）PEI25，000的鹽酸鹽形式，具更高的水溶特性；3）含更長(zhǎng)連續(xù)性乙烯亞胺（ethyleneimine）片段，其質(zhì)子化氮水平比PEI25，000提高11%以上。本品經(jīng)堿中和后，可轉(zhuǎn)化為線性化自由胺（PEI），此時(shí)分子量正相當(dāng)于25,000。
Thomas M，et al（2005）研究數(shù)據(jù)表明，去乙酰化的PEI 40,000（Polyethylenimine MAX 40,000）體外質(zhì)粒DNA轉(zhuǎn)染效率提高21倍，小鼠體內(nèi)靶向效率達(dá)到10,000倍，隨之肺部特異療效顯示增強(qiáng)1500倍。
化學(xué)性質(zhì)：
CAS NO. | 9002-98-6, 26913-06-4 |
Molecular Weight | 40,000（~22,000 free base） |
Solubility | Soluble In: Cold and room temperature water Insoluble In: Common organic solvents (ethanol, acetone, tetrahydrofuran) |
Appearance | White to off-white free flowing solid |
訂購(gòu)信息：【*，現(xiàn)貨供應(yīng)】
品牌 | 貨號(hào) | 名稱 | 規(guī)格 | *（元） |
Polysciences | 24765-2 | 線性聚乙烯亞胺鹽酸鹽Max，（MW 40，000） (Equivalent to Mw 25,000 in Free Base Form) | 2g | 3184 |
另，提供基本型PEI 25，000。 | ||||
Polysciences | 23966-2 | 線性聚乙烯亞胺（MW 25，000） | 2g | 2684 |
應(yīng)用文獻(xiàn)【基因轉(zhuǎn)染用途】 ：
Mann JF, McKay PF, Arokiasamy S, Pa RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
——Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】